Western Blot
Western blots remain the foremost biochemical method for protein analysis. The specificity of the Western blot protocol makes it ideal for protein quantitation.
Our Western blot services provide robust and flexible options for detecting your protein of interest in a variety of sample types. Customized Western blot workflows can accommodate your experimental needs.
Variable Gel Chemistry
Gel chemistry can be modified to suit your needs. Native, denaturing, and gradient gels are easily incorporated into our Western blot services.
Antibody Options Available
Our existing collection of vetted primary and secondary antibodies are available for use. We gladly utilize client-supplied reagents as well.
Co-Immunoprecipitation Studies
Probe protein interactions with expertly executed co-immunoprecipitation (co-IP) studies. Co-IPs can characterize known interacting partners, or identify novel interacting proteins.
Western Blots of Subcellular Fractions
To determine intracellular localization, cell lysates can be fractionated and analyzed as part of your Western blot workflow.
Sample Flexibility
Our existing collection of vetted primary and secondary antibodies are available for use. We gladly utilize client-supplied reagents as well.
Our Western blot services are not limited to a standard protocol. We provide many variations as part of our full-service biochemical assay offerings. These include total protein analyses, dot blots, native gel chemistry, zymograms, interaction studies, protein band isolation, recombinant protein production and analysis, and more. Wan Care can integrate these analyses into a larger experimental workflow to optimize project workflow and reduce experimental timelines.